Using bromothymol blue you wouldn't be able to distinquish between anacid with pH 4 from an acid with pH 5. Determination of the pK_a of Bromothymol Blue Use the following graph to determine the molar absorptivity of Bromothymol blue (BTB)? Spectrophotometric determination of the pKa of Bromothymol Blue. Computational methods, such as SQUAD (84) 16 and SPECFIT/32, and some new methods Favorite Answer. Molecular Weight 646.36 . NACRES NA.47 Anonymous. There is a linear relationship between log ( [ln-]/ [Hln]) and pH. Bromothymol Blue sodium salt powder Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt CAS Number 34722-90-2. The pKa′ value was determined using a method similar to that of Reilley and Swayer. 2. The pKa can be determined from the spectrophotometric data using different methods. EC Number 252-169-7. It shows that pH=pKa where [ln-]= [Hln] or hwere ( [ln-]/ [Hln])=1 or where log ( [ln-]/ [Hln])=0. Assume a cuvet with a 14.45 mm diameter was used. Molar absorptivity ___ (You will be using this value in your experiment.) Acid - Base indicators are either weak acids or weak bases so they exist as molecules in equilibrium with their ions. Other indicators work in different pH ranges. Description: Bromothymol Blue (BTB) is a pH indicator (pKa approx. ,IC10113005EA,IC10113025EA. Empirical Formula (Hill Notation) C 27 H 27 Br 2 NaO 5 S . Supplier: MP Biomedicals. pH = pKa - log ( [HIn] / [In-] ) … (2) They would both appearyellow. Relevance. Bromothymol blue, Powder ACS. Solutions of different concentration of the indicator were used. As a pH indicator,bromothymol blue, for example, would be useful between from about pH6.0 to pH 7.6. Answer Save. The indicator (HIn) is a monoprotic acid and we can represent its dissociation as follows: HIn → H+ + In- … (1) The equilibrium expression for such dissociation can be written as. [10] [11] describe multiwavelength analysis and nonlinear least squares regres-sion methods. Place three small drops of bromothymol blue solution into each of the 5 cuvettes. In this experiment, spectrophotometry is employed to measure the pKa of bromothymol blue, an acid-base indicator. 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the mic... SDS. 1 decade ago. Meloun et al. Given the equation: y= 48048x- 0.0105, find the molar absorptivity and use this value in your experiment. Explain why the acid - base indicator bromothymol blue turns yellow when added to dilute HCl but blue when added to dilute NaOH. Bromothymol blue is an acidic pH chromoionophore where acid to base change corresponds to its neutral to anionic form, respectively. Beilstein/REAXYS Number 6887926 . For this reason it can be electrostatically immobilized on the surface on anion-exchanger resin like Amberlite IRA 401. MDL number MFCD00077263. 2 Answers. Chloroform showed the highest affinity for bromothymol blue, followed by methylene chloride, ethylene dichloride, benzene, toluene, and carbon tetrachloride. Indicator: pH Range: pKa: Acid Form: Base Form: Methy Violet: 0.0 - 1.6: 0.8: Yellow: Blue: Thymol Blue: 1.2 - 2.8: 1.6: Red: Yellow: Methyl Yellow: 29. Into each of the cuvettes place 3 mL of the solutions of the various pH values (hydrochloric acid, the buffer solutions with pH values 6.0, 7.0 and 8.0, and sodium hydroxide solution). PubChem Substance ID 24892083. Ensure that the solutions are mixed well. 27 Br 2 NaO 5 S walls or nuclei under the mic....... Ph 5 an acid with pH 4 from an acid with pH 5 sodium CAS! Pk_A of bromothymol blue use the following graph to determine the molar absorptivity ___ ( You will using... This value in your experiment. by methylene chloride, ethylene dichloride, benzene, toluene, and tetrachloride. 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